rabbit anti human phospho ezrin antibody  (Cell Signaling Technology Inc)

Journal: BMC Cancer

Article Title: Promotion of the occurrence of endometrioid carcinoma by S100 calcium binding protein P

doi: 10.1186/s12885-020-07350-x

Figure Lengend Snippet: The colocalization of S100P and Ezrin, and F-actin rearrangement after knocking down S100P . A: Laser confocal microscopy showing the colocalization (yellow) of S100P (green) and Ezrin (red) in RL95–2 cells. The panels below (e, f, g, h) are an enlargement of the box area (a, b, c, d). B and C: FITC-phalloidin (green) staining showing the rearrangement of F-actin in RL95–2 cells (left) and Ishikawa cells (right). Scramble control (a, c, e) or S100P shRNA (b, d, f) set as mentioned previously. The nuclei were counterstained using Hoechst33258 (blue). The magnification of (A) is displayed using a ruler and the magnification in B and C was 400 ×

Article Snippet: After permeabilization with 0.1% Triton X-100 for 45 min, rabbit anti-human Ezrin 1: 50 dilution (Epitomics, Burlingame, CA, USA; EP886Y) and mouse anti-human S100P 1:50 dilution (R&D Systems) were used as the primary antibodies.

Techniques: Confocal Microscopy, Staining, Control, shRNA